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KMID : 1149220120200020037
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Korean Journal of Oriental Medical Prescription
2012 Volume.20 No. 2 p.37 ~ p.46
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Simultaneous Analysis of Six Constituents in Oyaksungi-san using HPLC-PDA
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Seo Chang-Seob
Kim Jung-Hoon
Shin Hyeun-Kyoo
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Abstract
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Objectives : Oyaksungi-san(Wuyaoshunqisan) has been used for treatment of stroke and rheumatoid arthritis in Korea. In this study, a simple and accurate high-performance liquid chromatography(HPLC) method was established for simultaneous determination of six main components, liquiritin, ferulic acid, naringin, hesperidin, neohesperidin, and glycyrrhizin in Oyaksungi-san, a traditional Korean herbal prescription.
Methods : The analytical column for separation of six constituents was used a Gemini C18 column maintained at 40℃. The mobile phase consisted of two solvent systems, 1.0% (v/v) acetic acid in H2O (A) and 1.0% (v/v) acetic acid in acetonitrile (B) by gradient flow. The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 254 nm for glycyrrhizin, 280 nm for liquiritin, naringin, hesperidin, and neohesperidin, and 320 nm for ferulic acid.
Results : Calibration curves were acquired with r2 values≥ 0.9998. The results of recovery test were 91.58%-105.90% with a relative standard deviations (RSDs, %) value less than 2.0%. The values of RSD for intra- and inter-day precision were 0.03%-1.72% and 0.03%-1.63%, respectively. The contents of the six compounds in Oyaksungi-san were 0.33-9.30 mg/g.
Conclusions : The newly established HPLC method will be helpful to improve quality control of Oyaksungi-san.
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KEYWORD
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Simultaneous analysis, Oyaksungi-san(Wuyaoshunqisan), HPLC-PDA, method validation
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